Calculadora de Cinética Enzimática (Michaelis-Menten) Gratis
Calcula Vmax y Km usando el modelo de Michaelis-Menten para cinética enzimática.
Reaction Velocity (v)
33.33 umol/min
Reaction Velocity (v) vs Substrate Concentration [S]
Fórmula
## Michaelis-Menten Enzyme Kinetics The Michaelis-Menten equation describes the rate of enzyme-catalyzed reactions as a function of substrate concentration. ### Formula **v = Vmax × [S] / (Km + [S])** where Vmax is the maximum velocity, Km is the substrate concentration at half-Vmax, and [S] is the substrate concentration. When [S] = Km, v = Vmax/2. When [S] >> Km, v approaches Vmax.
Ejemplo Resuelto
An enzyme with Vmax = 100 umol/min and Km = 50 uM at [S] = 25 uM.
- 01v = 100 × 25 / (50 + 25)
- 02v = 2500 / 75 = 33.33 umol/min
- 03v/Vmax = 25/75 = 0.333 (33.3% of Vmax)
Preguntas Frecuentes
What does Km represent biologically?
Km is the substrate concentration at which the enzyme operates at half its maximum rate. Low Km means high affinity (enzyme is efficient at low [S]). Typical Km values range from 0.1 uM to 10 mM.
When does the Michaelis-Menten model fail?
It fails for allosteric enzymes (sigmoidal kinetics), multi-substrate reactions, substrate inhibition, cooperative binding, and when the steady-state assumption does not hold.
How are Vmax and Km determined experimentally?
Measure initial velocities at several substrate concentrations. Plot v vs [S] for a direct fit, or use linearized plots (Lineweaver-Burk, Eadie-Hofstee, Hanes-Woolf) to extract the parameters.
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