Calculadora de Resolução Cromatográfica — Formula
## Chromatographic Resolution
Resolution quantifies how well two adjacent peaks are separated. It combines the effects of selectivity, efficiency, and retention.
### Formula
**Rs = 2 × (tR2 - tR1) / (w1 + w2)**
where tR is retention time and w is peak width at the base (4-sigma width for Gaussian peaks).
- Rs < 1.0: peaks overlap significantly
- Rs = 1.0: about 4% overlap
- Rs = 1.5: baseline separation (< 0.3% overlap)
- Rs >= 2.0: complete separation
Resolution quantifies how well two adjacent peaks are separated. It combines the effects of selectivity, efficiency, and retention.
### Formula
**Rs = 2 × (tR2 - tR1) / (w1 + w2)**
where tR is retention time and w is peak width at the base (4-sigma width for Gaussian peaks).
- Rs < 1.0: peaks overlap significantly
- Rs = 1.0: about 4% overlap
- Rs = 1.5: baseline separation (< 0.3% overlap)
- Rs >= 2.0: complete separation
Exemplo Resolvido
Two peaks at 5.2 and 5.8 min with base widths of 0.30 and 0.35 min.
- Rs = 2 × (5.8 - 5.2) / (0.30 + 0.35)
- Rs = 2 × 0.6 / 0.65 = 1.2 / 0.65 = 1.846
- Baseline separation achieved (Rs > 1.5)