Calculadora de Resolução Cromatográfica
Calcule a resolução entre picos cromatográficos para avaliar a eficiência da separação.
Resolution (Rs)
1.846
Resolution (Rs) vs Retention Time Peak 1 (tR1)
Formula
## Chromatographic Resolution Resolution quantifies how well two adjacent peaks are separated. It combines the effects of selectivity, efficiency, and retention. ### Formula **Rs = 2 × (tR2 - tR1) / (w1 + w2)** where tR is retention time and w is peak width at the base (4-sigma width for Gaussian peaks). - Rs < 1.0: peaks overlap significantly - Rs = 1.0: about 4% overlap - Rs = 1.5: baseline separation (< 0.3% overlap) - Rs >= 2.0: complete separation
Exemplo Resolvido
Two peaks at 5.2 and 5.8 min with base widths of 0.30 and 0.35 min.
- 01Rs = 2 × (5.8 - 5.2) / (0.30 + 0.35)
- 02Rs = 2 × 0.6 / 0.65 = 1.2 / 0.65 = 1.846
- 03Baseline separation achieved (Rs > 1.5)
Perguntas Frequentes
What resolution is needed for quantitation?
Rs >= 1.5 (baseline separation) is required for accurate quantitation. For screening purposes, Rs >= 1.0 may suffice. Pharmacopeial methods often require Rs >= 2.0.
How can I improve resolution?
Three approaches: increase selectivity (change mobile phase, stationary phase, or temperature), increase efficiency (use longer column or smaller particles), or increase retention (change mobile phase strength). Selectivity changes are most effective.
How does resolution scale with column length?
Rs is proportional to sqrt(N), where N is the plate count. Doubling the column length doubles N but only improves Rs by sqrt(2) = 1.41×. This also doubles run time and back pressure.
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