Calcolatore Decadimento Radioattivo Avanzato — Formula
## Bradford Protein Assay
The Bradford assay uses Coomassie Brilliant Blue G-250 dye, which shifts from red to blue upon binding protein. Absorbance at 595 nm is proportional to protein concentration.
### Calculation
**[Protein] = (A595 - b) / m × dilution factor**
where m and b come from a standard curve using BSA or another reference protein. The Bradford assay is sensitive to 1-20 ug/mL but has protein-to-protein variation.
The Bradford assay uses Coomassie Brilliant Blue G-250 dye, which shifts from red to blue upon binding protein. Absorbance at 595 nm is proportional to protein concentration.
### Calculation
**[Protein] = (A595 - b) / m × dilution factor**
where m and b come from a standard curve using BSA or another reference protein. The Bradford assay is sensitive to 1-20 ug/mL but has protein-to-protein variation.
Esempio Risolto
A Bradford assay reads A595 = 0.45, standard curve: m = 0.05, b = 0.02, no dilution.
- [Protein] = (0.45 - 0.02) / 0.05 × 1
- [Protein] = 0.43 / 0.05 = 8.60 ug/mL