DNA गलनांक कैलकुलेटरसूत्र

## Michaelis-Menten Enzyme Kinetics

The Michaelis-Menten equation describes the rate of enzyme-catalyzed reactions as a function of substrate concentration.

### Formula

**v = Vmax × [S] / (Km + [S])**

where Vmax is the maximum velocity, Km is the substrate concentration at half-Vmax, and [S] is the substrate concentration. When [S] = Km, v = Vmax/2. When [S] >> Km, v approaches Vmax.

हल किया गया उदाहरण

An enzyme with Vmax = 100 umol/min and Km = 50 uM at [S] = 25 uM.

  1. v = 100 × 25 / (50 + 25)
  2. v = 2500 / 75 = 33.33 umol/min
  3. v/Vmax = 25/75 = 0.333 (33.3% of Vmax)