प्रोटीन सांद्रता (ब्रैडफोर्ड) कैलकुलेटर — सूत्र

## Enzyme Inhibition Types

### Competitive Inhibition
Inhibitor binds to the active site, increasing apparent Km while Vmax remains unchanged.

**v = Vmax × [S] / (alpha × Km + [S])**, where alpha = 1 + [I]/Ki

### Noncompetitive Inhibition
Inhibitor binds away from the active site, decreasing apparent Vmax while Km remains unchanged.

**v = (Vmax/alpha) × [S] / (Km + [S])**

Competitive inhibition can be overcome by high substrate concentration; noncompetitive cannot.

हल किया गया उदाहरण

Vmax=100, Km=50 uM, [I]=20 uM, Ki=10 uM, [S]=50 uM.

  1. alpha = 1 + 20/10 = 3.0
  2. Competitive: v = 100 × 50 / (50 × 3 + 50) = 5000 / 200 = 25.0 umol/min
  3. Noncompetitive: v = (100/3) × 50 / (50 + 50) = 33.33 × 0.5 = 16.67 umol/min