Calculateur de Profil Isotopique — Formule
## Retention Factor (Capacity Factor)
The retention factor k' describes how strongly a compound is retained by the stationary phase relative to the mobile phase.
### Formula
**k' = (tR - t0) / t0**
where tR is the retention time of the analyte and t0 is the void (dead) time. Ideal k' values are 2-10. Below 1, peaks elute near the void and separation is poor. Above 20, peaks are broad and run time is long.
The retention factor k' describes how strongly a compound is retained by the stationary phase relative to the mobile phase.
### Formula
**k' = (tR - t0) / t0**
where tR is the retention time of the analyte and t0 is the void (dead) time. Ideal k' values are 2-10. Below 1, peaks elute near the void and separation is poor. Above 20, peaks are broad and run time is long.
Exemple Résolu
A peak at tR = 8.5 min with void time t0 = 2.0 min.
- k' = (8.5 - 2.0) / 2.0 = 6.5 / 2.0 = 3.25
- The compound spends 3.25 times more time in the stationary phase than the mobile phase.