Calculateur de Cinétique Enzymatique — Formule
## Theoretical Plate Number
The plate count N measures column efficiency. Higher N means sharper peaks and better separation potential.
### Formula
**N = 16 × (tR / w)²**
**HETP = L / N**
where w is the peak width at the base (4-sigma). Modern HPLC columns typically give N = 10,000-20,000 per 25 cm column. UHPLC with sub-2-um particles can achieve 30,000+ plates.
The plate count N measures column efficiency. Higher N means sharper peaks and better separation potential.
### Formula
**N = 16 × (tR / w)²**
**HETP = L / N**
where w is the peak width at the base (4-sigma). Modern HPLC columns typically give N = 10,000-20,000 per 25 cm column. UHPLC with sub-2-um particles can achieve 30,000+ plates.
Exemple Résolu
A peak at 10 min with 0.25 min base width on a 25 cm column.
- N = 16 × (10 / 0.25)² = 16 × 40² = 16 × 1600 = 25,600 plates
- HETP = 25 / 25600 = 0.000977 cm = 9.8 um