Calculateur de Facteur de Rétention — Formule
## Linear Calibration Curve
A calibration curve relates instrument signal to analyte concentration. With a linear model y = mx + b, an unknown concentration is found by inverting this relationship.
### Formula
**c_unknown = (y_measured - b) / m**
where y is the measured signal, m is the slope from standard curve regression, and b is the y-intercept. The calibration curve should be established from at least 5 standard concentrations spanning the expected range.
A calibration curve relates instrument signal to analyte concentration. With a linear model y = mx + b, an unknown concentration is found by inverting this relationship.
### Formula
**c_unknown = (y_measured - b) / m**
where y is the measured signal, m is the slope from standard curve regression, and b is the y-intercept. The calibration curve should be established from at least 5 standard concentrations spanning the expected range.
Exemple Résolu
A calibration with slope = 2500 (signal/M), intercept = 0.05, and measured signal = 1.3.
- c = (1.3 - 0.05) / 2500
- c = 1.25 / 2500 = 0.000500 M = 0.500 mM