Free Bradford Assay Calculator
Calculate protein concentration from Bradford assay absorbance using a linear calibration curve.
Protein Concentration
8.60 ug/mL
Protein Concentration vs Absorbance at 595 nm
Bradford Protein Assay
The Bradford assay uses Coomassie Brilliant Blue G-250 dye, which shifts from red to blue upon binding protein. Absorbance at 595 nm is proportional to protein concentration.
Calculation
[Protein] = (A595 - b) / m × dilution factor
where m and b come from a standard curve using BSA or another reference protein. The Bradford assay is sensitive to 1-20 ug/mL but has protein-to-protein variation.
Example Calculation
A Bradford assay reads A595 = 0.45, standard curve: m = 0.05, b = 0.02, no dilution.
- 01[Protein] = (0.45 - 0.02) / 0.05 × 1
- 02[Protein] = 0.43 / 0.05 = 8.60 ug/mL
Frequently Asked Questions
Learn More
Understanding Molarity
Learn what molarity is, how to calculate it, and why it matters in chemistry. Covers moles, liters, dilution, and step-by-step examples for preparing solutions.