Free Michaelis-Menten Calculator
Calculate the reaction velocity for an enzyme-catalyzed reaction using Michaelis-Menten kinetics.
Reaction Velocity (v)
33.33 umol/min
Reaction Velocity (v) vs Substrate Concentration [S]
Michaelis-Menten Enzyme Kinetics
The Michaelis-Menten equation describes the rate of enzyme-catalyzed reactions as a function of substrate concentration.
Formula
v = Vmax × [S] / (Km + [S])
where Vmax is the maximum velocity, Km is the substrate concentration at half-Vmax, and [S] is the substrate concentration. When [S] = Km, v = Vmax/2. When [S] >> Km, v approaches Vmax.
Example Calculation
An enzyme with Vmax = 100 umol/min and Km = 50 uM at [S] = 25 uM.
- 01v = 100 × 25 / (50 + 25)
- 02v = 2500 / 75 = 33.33 umol/min
- 03v/Vmax = 25/75 = 0.333 (33.3% of Vmax)
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