Free Lineweaver-Burk Calculator
Calculate the Lineweaver-Burk (double reciprocal) plot values from Vmax and Km for enzyme kinetics linearization.
Y-Intercept (1/Vmax)
0.010000
Y-Intercept (1/Vmax) vs Vmax
Lineweaver-Burk Plot
The Lineweaver-Burk (double reciprocal) plot linearizes the Michaelis-Menten equation by plotting 1/v vs 1/[S].
Formula
1/v = (Km/Vmax) × (1/[S]) + 1/Vmax
This is a straight line with slope = Km/Vmax, y-intercept = 1/Vmax, and x-intercept = -1/Km. While historically important, nonlinear regression is preferred today because Lineweaver-Burk overweights low-concentration data.
Example Calculation
An enzyme with Vmax = 100 umol/min and Km = 50 uM.
- 01Y-intercept = 1/100 = 0.01
- 02Slope = 50/100 = 0.5
- 03X-intercept = -1/50 = -0.02
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