Calculadora de Lineweaver-Burk — Formula
## Lineweaver-Burk Plot
The Lineweaver-Burk (double reciprocal) plot linearizes the Michaelis-Menten equation by plotting 1/v vs 1/[S].
### Formula
**1/v = (Km/Vmax) × (1/[S]) + 1/Vmax**
This is a straight line with slope = Km/Vmax, y-intercept = 1/Vmax, and x-intercept = -1/Km. While historically important, nonlinear regression is preferred today because Lineweaver-Burk overweights low-concentration data.
The Lineweaver-Burk (double reciprocal) plot linearizes the Michaelis-Menten equation by plotting 1/v vs 1/[S].
### Formula
**1/v = (Km/Vmax) × (1/[S]) + 1/Vmax**
This is a straight line with slope = Km/Vmax, y-intercept = 1/Vmax, and x-intercept = -1/Km. While historically important, nonlinear regression is preferred today because Lineweaver-Burk overweights low-concentration data.
Exemplo Resolvido
An enzyme with Vmax = 100 umol/min and Km = 50 uM.
- Y-intercept = 1/100 = 0.01
- Slope = 50/100 = 0.5
- X-intercept = -1/50 = -0.02