Calculadora da Lei de Beer-Lambert — Formula
## Beer-Lambert Law
The Beer-Lambert law relates the absorbance of a solution to the concentration of the absorbing species, the path length, and the molar absorptivity.
### Formula
**A = epsilon × l × c**
where A is absorbance (dimensionless), epsilon is the molar absorptivity (L/(mol·cm)), l is the optical path length (cm), and c is the molar concentration (mol/L). Transmittance: T = 10^(-A).
The Beer-Lambert law relates the absorbance of a solution to the concentration of the absorbing species, the path length, and the molar absorptivity.
### Formula
**A = epsilon × l × c**
where A is absorbance (dimensionless), epsilon is the molar absorptivity (L/(mol·cm)), l is the optical path length (cm), and c is the molar concentration (mol/L). Transmittance: T = 10^(-A).
Exemplo Resolvido
NADH in a 1 cm cuvette at 0.5 mM concentration (epsilon = 6220 at 340 nm).
- A = 6220 × 1 × 0.0005 = 3.110
- %T = 10^(-3.110) × 100 = 0.0776 × 100 = 7.76%