Free Peak Asymmetry Calculator
Calculate the asymmetry factor (tailing factor) of a chromatographic peak at 10% peak height.
mm
mm
Asymmetry Factor (As)
1.200
USP Tailing Factor (Tf)1.100
Asymmetry Factor (As) vs Back Half-Width at 10% Height (B)
Peak Asymmetry and Tailing Factor
Peak shape is a critical quality attribute in chromatography. Asymmetric peaks indicate column problems or unwanted secondary interactions.
Formulas
Asymmetry Factor: As = B / A (measured at 10% height)
USP Tailing Factor: Tf = (A + B) / (2A) (measured at 5% height, but often approximated at 10%)
Example Calculation
A peak with front half-width A = 5 mm and back half-width B = 6 mm at 10% height.
- 01As = 6 / 5 = 1.200
- 02Tf = (5 + 6) / (2 × 5) = 11 / 10 = 1.100
- 03Slight tailing (acceptable; As < 1.5 is typically OK)
Frequently Asked Questions
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